Single-molecule approach to dispersed kinetics and dynamic disorder: Probing conformational fluctuation and enzymatic dynamics
نویسنده
چکیده
This article reviews our efforts in understanding dynamical fluctuations of both conformation and enzymatic reactivity in single biomolecules. The single-molecule approach is shown to be particularly powerful for studies of dispersed kinetics and dynamic disorder. New single-molecule observations have revealed conformational transitions occurring on a broad range of timescales, 100 ms–10 s, offering new clues for understanding energy landscape of proteins, as well as the structural and chemical dynamics therein. © 2002 American Institute of Physics. @DOI: 10.1063/1.1521159#
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تاریخ انتشار 2002